The incorporation of l-asparagine-14C by lymphoma 6C3HED cells: its inhibition by guinea pig serum.

نویسندگان

  • J G Kidd
  • L H Sobin
چکیده

was obtained with normal horse serum, which does not have antilymphoma effects and is known to be devoid of asparaginase. By contrast, under identical conditions in vivo, the 6C3HED cells failed to take up L-aspartic acid-14C,or did so only poorly. In vitro, the Lymphoma 6C3HED-OG cells also took up Lasparagine-14C avidly. Again, the incorporation of L-asparagineJ4C was almost completely prevented by guinea pig serum, either unheated or heated at 56°Cfor 30 min, whereas horse

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The Incorporation of L-Asparagine-14C by Lymphoma 6C3HED Cells: Its Inhibition by Guinea Pig Serum1

was obtained with normal horse serum, which does not have antilymphoma effects and is known to be devoid of asparaginase. By contrast, under identical conditions in vivo, the 6C3HED cells failed to take up L-aspartic acid-14C,or did so only poorly. In vitro, the Lymphoma 6C3HED-OG cells also took up Lasparagine-14C avidly. Again, the incorporation of L-asparagineJ4C was almost completely preven...

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In Vivo and in Vitro Effects of the L-Asparaginase Fraction of Guinea Pig Serum on the 6C3HED Ascites Lymphosarcoma1

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In vivo and in vitro effects of the L-asparaginase fraction of guinea pig serum on the 6C3HED ascites lymphosarcoma.

The antitumor activity of guinea 1'ig serum fractions was studied in vivo and in vitro by bright field, fluorescence, and electron microscopy observations and by dye permeability and cytidine-3H incorporation in 6C3HED lymphosarcoma cells. In C3H mice bearing the ascites tumor treated with the Lasparaginase fraction, there was a decrease in the total num ber and mitosis of tumor cells, a reduct...

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Evidence That the L-asparaginase of Guinea Pig Serum Is Responsible for Its Antilymphoma Effects

A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially abs...

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عنوان ژورنال:
  • Cancer research

دوره 26 2  شماره 

صفحات  -

تاریخ انتشار 1966